Ultrastructural injury to human spermatozoa after freezing and thawing
نویسندگان
چکیده
منابع مشابه
Evaluation of human sperm function after repeated freezing and thawing.
Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to sperm after repeated freezing and thawing. The results showed that normozoospermic and oligozoospermic sperm survive...
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Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of freezing and thawing, special care must taken. Here we demonstrate the proper technique for rapidl...
متن کاملP-65: Low-Density Lipoproteins Extractions on Boar Spermatozoa Quality Following Freezing–Thawing
Background Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. Ma...
متن کامل“Short Paper” Application of Gamma Irradiation to Increase the Motility and Viability of Bovine Sperm after Freezing and Thawing
This study was conducted to determine the suitable dose of gamma rays for increasing sperm motility and viability after thawing and to assess its effects on malondialdehyde concentration and the rate of sperm DNA strand breaks. Sperm samples in liquid nitrogen were irradiated at doses of zero, 0.1, 0.3, 0.5, 0.7 and 0.9 Gy. The sperm quality and quantity parameters were evaluated before and aft...
متن کاملThe Efficacy of Glucose and Electrolyte Solutions in Preserving the Structure of Fowl Spermatozoa after Freezing and Thawing
HARRIS et al.1), observing ultrastructural changes in rapidly frozen fowl spermatozoa, noted destructive effects on the acrosome, the midpiece, and the cytoplasmic membrane. Similar ultrastructural damages were seen by BAKST and SEXTON2) in frozen-thawed fowl and turkey spermatozoa under transmission and scanning electron microscopes. However, they gave little information on the possible mechan...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1978
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0530389